Journal: bioRxiv

Article Title: Compromised striatal structure and function in mouse models of RARB-related disorder

doi: 10.64898/2026.02.20.706764

Figure Lengend Snippet: (A,B) Bodyweight of 20-week-old males (A) and females (B). ( C) Examples of eyes from 20-week-old mice. Red arrows indicate pupil. (D,E) Evaluation of eye ball size (D) and (E) pupil size. (F) Novelty induced locomotor activity in the open field (n=10 for each line). (G) Quantification of the number of rears in the center of the open field overtime. (H) Mean latency to fall from the rotating cylinder in the accelerated rotarod paradigm. (I) Spontaneous locomotor activity was measured in actimetric cages over 32 h (n=10 for all genotypes). (J) Spontaneous locomotor activity was measured in actimetric cages during 12 hours of the dark period. (K) Short-term declarative memory was assessed in novel object recognition assay. Recognition index was calculated and plotted. Orange line indicates at chance performance. Statistical differences were calculated using Bonferroni multiple comparisons as post-hoc follow-up of ANOVA and indicate: *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars represent SEM.

Article Snippet: The test was performed in an open-field arenas described above (Panlab, France).

Techniques: Activity Assay

Journal: bioRxiv

Article Title: Compromised striatal structure and function in mouse models of RARB-related disorder

doi: 10.64898/2026.02.20.706764

Figure Lengend Snippet: (A) Quantification of the total number of rears during 30 minutes of the open field test. (B) Quantification of the total number of rears in the center of the open field arena for 30 minutes. (C) Evaluation of the percentage of time spent in the center of arena for 30 minutes (note that no difference was observed during the first 5 minutes of the test). (D) Motor coordination was assessed as number of slips on a 1 meter notched bar and it was represented as mean of 3 trials. (E) Muscular strength measurements were assessed as mean of grip strength corrected for body weight or (F) by measuring hanging time of mice on a inverted grid. (G) Anxiety was evaluated in elevated plus maze by measuring the time spent in anxiogenic open arms and (H) the elapsed time before the first entry in anxiogenic open arms. (I) Quantification of the total number of rears during 30 minutes in the center of open field arena for Rarb +/- mice. (J) Spontaneous locomotor activity was measured in actimetric cages during 12 hours of the dark cycle for Rarb +/- mice. (K) Rarb +/- mice motor coordination was evaluated in accelerated rotarod as mean latency to fall from the rotating cylinder. (L) Rarb +/- mice short-term declarative memory was assessed with novel object recognition test. Recognition index was then calculated and plotted, and orange line indicates at chance performance level. Statistical differences were calculated using Bonferroni multiple comparisons as post-hoc follow-up of ANOVA analyses or Mann-Whitney test and indicate: *, p < 0.05; * *, p < 0.01; * **, p < 0.001. Error bars represent SEM.

Article Snippet: The test was performed in an open-field arenas described above (Panlab, France).

Techniques: Activity Assay, MANN-WHITNEY

Journal: bioRxiv

Article Title: Compromised striatal structure and function in mouse models of RARB-related disorder

doi: 10.64898/2026.02.20.706764

Figure Lengend Snippet: (A) Body weight progression between 1 and 5 weeks of age. (B) Body weight progression between 1- and 5-week-old females. (C). Quantification of the total number of rears during 30 minutes in the center of open field arena. (D) Progression of motor coordination deficits in the accelerated rotarod was expressed as mean latency to fall from the rotating cylinder. (E) Quantification of the number of slips on a 1 meter notched bar in 3- and 5-week-old mice. (F) Spontaneous locomotor activity of 5-week-old mice was measured in actimetric cages over 32 hours including 12hrs of light/dark cycle (n = 7/genotype). (G) Spontaneous locomotor activity in actimetric cages during the dark period was compared at 5 and 12 weeks of age. (H) Short-term declarative memory was assessed with novel object recognition assay at 8 and 12 weeks of age. Recognition index was calculated and plotted. Dashed orange line indicates at chance performance. Statistical differences were calculated using Bonferroni multiple comparisons as post-hoc follow-up of ANOVA analyses and indicate: *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars represent SEM.

Article Snippet: The test was performed in an open-field arenas described above (Panlab, France).

Techniques: Activity Assay

Journal: bioRxiv

Article Title: Compromised striatal structure and function in mouse models of RARB-related disorder

doi: 10.64898/2026.02.20.706764

Figure Lengend Snippet: (A) Novelty induced locomotor activity in open field evaluated 30 minutes after intra-peritoneal injection of 0.2 mg/kg (mpk) or 2 mpk of pramipexol. (B) Quantification of the number of rears in the center of the open field 30 minutes after intra-peritoneal injection of 0.2 mpk or 2 mpk of pramipexol. (C) Motor coordination was evaluated in accelerated rotarod as mean latency to fall from the rotating cylinder 45 minutes after intra-peritoneal injection of 0.2 mpk or 2 mpk of pramipexol. (D) Long term effect of pramipexol on night spontaneous activity measured in actimetric cages. Pramipexol was injected at the onset of the dark period (7pm) after 8 hours of habituation in actimetric cages. Statistical differences were calculated using Bonferroni multiple comparisons as post-hoc follow-up of two-way ANOVA analyses and indicate: *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars represent SEM.

Article Snippet: The test was performed in an open-field arenas described above (Panlab, France).

Techniques: Activity Assay, Injection